Development and Validation of SYBR Green-Based qPCR Technique of Detection and Quantification of <i>Salmonella enterica</i>

Salmonella enterica serovars is a leading cause of human gastroenteritis, and the incidence of salmonellosis constantly increasing, causing millions infections and many deaths annually. The detection pathogen in optimal terms an essential factor for reducing impact on body. In this work, SYBR Green I-based qPCR method quantification was developed validated. For subsp. enterica, two pairs primers were designed using publically available Primer-BLAST software. Primer efficiency calculated by establishing standard curve. specificity, sensitivity, accuracy, precision PCR results tested. Both primer pairs showed an acceptable performance, proving techniques were sensitive, reliable precise. validated technology has good potential to replace traditional culture method microbial diagnosis.